Cardiac Cell Type Specific Surfaceomes
Cell surface proteins are often low abundance, hydrophobic proteins that are not well-represented in whole cell lysate-based proteomic analyses. They can be even more difficult to detect in tissue lysates, as when heterogeneous mixtures of cell types are analyzed simultaneously, it can result in low abundance signals being overwhelmed by the average of the higher abundance proteins.
For these reasons, our studies of the human heart begin with isolated myocytes and fibroblasts, a strategy which provides us with unparalleled specificity and sensitivity regarding which molecules occupy the surface of each cell type. Our new µCSC makes these efforts possible, as we can apply the approach to as few as 5 million isolated heart cells, providing a powerful approach for mapping the surfaceome of the heart in a cell-type specific manner - revealing proteins never before described in the heart!
Our current efforts focus on defining the cardiac surfaceome in a cell-type, chamber- and developmental-stage specific approach, and includes cells isolated from normal and diseased cardiac tissue. Our analyses yield new information regarding receptors and ion channels important for defining the cardiac cell landscape and function, new therapeutic targets for treatment of heart disease, and useful metrics for correlating hPSC-derivatives with their relevant human counterparts.