Stem Cell & Tissue Culture Laboratories

Stem Cell Culture Suite

The laboratory has expertise in culturing and differentiating human embryonic and induced pluripotent stem cells using state-of-the-art protocols for chemically defined, feeder-free conditions. Phenotypic and functional assessments of stem cells and their derivatives include flow cytometry, imaging, metabolic flux, calcium flux, and electrophysiology.

The stem cell culture suite features 140 sq feet of space dedicated to human pluripotent stem cell culturing and differentiation. This room houses two 4 ft. Thermo Scientific 1300 series class II type A2 laminar flow hoods; four New Brunswick Galaxy 170S CO2 incubators; Nikon Eclipse TS2 dissection microscope.

Tissue Dissection & Culture Suite

The laboratory has expertise in isolating cardiomyocytes and cardiac fibroblasts from fresh human tissue, including donor hearts and surgical discards.

The tissue dissection and culture suite features 250 sq feet of space dedicated to the isolation and culture of primary human cells. This room features two 4 ft. Thermo Scientific 1300 series class II type A2 laminar flow hoods; four Thermo Scientific Heracell VIOS 160i CO2 incubators; Olympus IX81 fluorescence microscope.

Our novel GENTIL method for primary human cardiomyocyte isolation uses a Leica VT1200S Vibratome automated tissue slicer followed by Curiox HT2000 Laminar Flow washing station to remove debris and non-cardiomyocytes.